Measurement of apolipoprotein B radioactivity in whole blood plasma by precipitation with isopropanol.

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Measurement of apolipoprotein B radioactivity in whole blood plasma by precipitation with isopropanol.

A method to measure apolipoprotein B radioactivity in whole blood plasma is described that is suitable for routine use in kinetic experiments in vivo. Radiolabeled apolipoprotein B is precipitated from plasma diluted 15- to 30-fold in the presence of carrier low density lipoproteins by 50% isopropanol. The amount of radioiodine in apoB is estimated from the difference between total radioiodine ...

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Isopropanol precipitation method for the determination of apolipoprotein B specific activity and plasma concentrations during metabolic studies of very low density lipoprotein and low density lipoprotein apolipoprotein B.

A method has been described for the measurement of apoB concentration and specific activity in very low density lipoprotein (VLDL) and low density lipoprotein (LDL) during metabolic studies. For measurement of specific activity, apoB was separated from other apolipoproteins and lipids by precipitation in, and subsequent washing with, isopropanol. For determination of apoB concentration, equal v...

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Purification of genomic DNA from human whole blood by isopropanol-fractionation with concentrated Nal and SDS.

Many applications of gene analysis call for rapid and efficient methods for isolation of genome DNA from whole blood and complex biological samples. Relating to these demands, many protocols have been published (1-6) . However, either the methods involves toxic organic extractions and/or skillful manipulations such as spooling of DNA with an inoculation loop and the like to separate DNA from th...

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VLDL apolipoprotein B determination in blood serum following precipitation of LDL with polyvinylsulphate.

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ژورنال

عنوان ژورنال: Journal of Lipid Research

سال: 1988

ISSN: 0022-2275

DOI: 10.1016/s0022-2275(20)38790-3